Biochem/physiol Actions
Effective concentration = 100 ng/ml as measured by induction of Smad2 phosphorylation in P19 mouse embryonal carcinoma cells.
General description
Recombinant, mouse growth differentiation factor 9 (GDF-9) consisting of amino acids 307-477 and expressed in Chinese Hamster Ovary cells. GDF-9 is an oocyte secreted paracrine factor, necessary for mammalian ovarian folliculogenesis. Mature mouse GDF-9 is 90% identical to the amino acid sequence of mature human GDF-9. GDF-9 as a paracrine factor is useful in granulosa cell proliferation and differentiation regulation.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Other Notes
Mottershead, D.G., et al. 2008. Mol. Cell. Endocrinol.283, 58.Gilchrist, R.B., et al. 2006. J. Cell. Sci.119, 3811.Mazerbourg, S., et al. 2004. Mol. Endocrinol.18, 653.Kaivo-Oja, N., et al. 2003. J. Clin. Endocrinol. Metab.88, 755.Roh, J.S., et al. 2003. Endocrinology144, 172.Vitt, U.A., et al. 2002. Biol. Reprod.67, 473.Dong, J., et al. 1996. Nature383, 531.McGrath, S.A., et al. 1995. Mol. Endocrinol.9, 131.McPherron, A.C. and S.J. Lee. 1992. J. Biol. Chem.268, 3444.
Packaging
10 µg in Glass bottle
Physical form
Lyophilized from 0.2 µm sterile-filtered HCl, BSA.
Reconstitution
Reconstitute to 100 µg/ml in 4 mM HCl containing 0.1% bovine serum albumin.
Warning
Toxicity: Standard Handling (A)
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