Biochem/physiol Actions

Effective concentration = 100 ng/ml as measured by induction of Smad2 phosphorylation in P19 mouse embryonal carcinoma cells.

General description

Recombinant, mouse growth differentiation factor 9 (GDF-9) consisting of amino acids 307-477 and expressed in Chinese Hamster Ovary cells. GDF-9 is an oocyte secreted paracrine factor, necessary for mammalian ovarian folliculogenesis. Mature mouse GDF-9 is 90% identical to the amino acid sequence of mature human GDF-9. GDF-9 as a paracrine factor is useful in granulosa cell proliferation and differentiation regulation.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Mottershead, D.G., et al. 2008. Mol. Cell. Endocrinol.283, 58.Gilchrist, R.B., et al. 2006. J. Cell. Sci.119, 3811.Mazerbourg, S., et al. 2004. Mol. Endocrinol.18, 653.Kaivo-Oja, N., et al. 2003. J. Clin. Endocrinol. Metab.88, 755.Roh, J.S., et al. 2003. Endocrinology144, 172.Vitt, U.A., et al. 2002. Biol. Reprod.67, 473.Dong, J., et al. 1996. Nature383, 531.McGrath, S.A., et al. 1995. Mol. Endocrinol.9, 131.McPherron, A.C. and S.J. Lee. 1992. J. Biol. Chem.268, 3444.

Packaging

10 µg in Glass bottle

Physical form

Lyophilized from 0.2 µm sterile-filtered HCl, BSA.

Reconstitution

Reconstitute to 100 µg/ml in 4 mM HCl containing 0.1% bovine serum albumin.

Warning

Toxicity: Standard Handling (A)